Proposal Of Collaboration For Establishment Of Kidney Transplantation Program
By:
Gamal Saadi
Mai Hassaballa

[I] Man Power

(1) Nephrologists: to include a consultant and minimum of 2 Junior trainees to be trained for a period of 3 months to get acquainted with:
- Preparation of Recipients and Donors
- Perioperative care
- Graft biopsies
- Immunosuppression and management of rejections
- Infections related to immunosuppression
- Other transplantation related complications
- Management of Hypertension, NODAT, Anemia, MBD, etc,
- Tx in selected cases as FSGS

Trainers: [Fees of group 2000 $ / month]
- Supervisor
- Consultant
- Coordinator
- Assistant

(2) Nurse: to include 3 nurses to be trained for a period of 3 months to get acquainted with:
- Postoperative care
- Infection control
- Fluid and electrolyte balance

Trainers: [Fees of group 600 $ / month]
- Head Tx nurse
- Attending nurse
- Infection control supervisor

(3) Pathologist: to include 2 pathologists (provided a trained technician is available) to be trained for a period of 2 months to get acquainted with:

- Acute and chronic Cell MR
- Acute and chronic AMR
- Drug Toxicity
- Recurrence of original disease
- Viral infections

For the pathology technician if not available:
• Processing
• Cutting
• Special stains
• Basics of immunohistochemistry
[Training fees additional 600 $ for 2 months training]

Trainer: [Fee of supervisor 2000 $]
- Nephropathology expert

(4) Clinical Pathologist: to include 2 clinical pathologists and 2 technicians to be trained for a period of 2 months to get acquainted with:
- Luminex
- SSO (Sequence Specific Oligotyping)
- PCR
- Lymphocytotoxicity
- Lymphocyte cross match
- HLA typing
- DSA Luminex testing
- Drug monitoring
- Virology antibodies and PCR
- ABO Rh and routine Laboratory

Course of 2 months 4 days per week including 8 lectures and practical work with follow up training including consumables for 1000 $
Trainers: [Fees of group 2500 $ for the 2 months course]
[Training technicians if included 500 $ for course]
[Total 4000 $ per course]

(5) Transplant surgeon: to include 2 surgeons to be trained for a period of 1 month to attend 12 cases

Trainers: [Fees of group 4000 $ / month]
- Donor Surgeon
- Recipient bed
- Vascular anastomosis
- Ureteric anastomosis

In addition to:
* Centre registration fees
* Licenses fees (Interior affairs, MOH, Syndicate)
* Accommodation expenses
* Flight tickets


[II] Facilities and Requirements

Laboratory
- Luminex 3D 1,800,000 LE
- 2 centrifuges 160,000 LE
- PCR machine for typing 100,000 LE
- Vortex
- 5 Micropipettes x 6000 = 30,000 LE
- Shaking water bath 18,000 LE
- Inverted microscope (Olympus) 100,000 LE
- Light microscope (Olympus) 25,000 LE
- Nanodrop ( for DNA) 100,000 LE
- Electrophoresis chamber 60,000 LE
Total = 2,393,000 LE
= 133,000 $
Pathology
- Rotary Microtome (Preference Shandon, Leica)
- Microscope – 5 opticals including x40 and x100 (Olympus, zeiss, leica)
- Special stains (PAS Masson Trichrome,Congo red)
- Set up for immunohistochemistry or
- [Immunofluorescence requires IF microscope, cryostat]
- Primary antibodies: IgG, IgA, C3, C4d


Radiology:
- Spiral CT
- Duplex ultrasonography

ICU

Extracorporeal Interventions:
- Hemodialysis
- Plasma Exchange

Operative Theater

Registry

[III] Activity Implementation